Diluent Buffer for Antibody
First Edition (Revised on April, 2016)
Diluent Buffer for Antibody is mainly used for antibody dilution. On one hand, it provids a suitable PH and ionic concentration to enhance specific binding capacity of antibody and antigen, and reduce nonspecific binding; on the other hand, protective agent of buffer can ensure stability of antibody.
REAGENTS AND MATERIALS PROVIDED
|Diluent Buffer for Antibody||1 bottle of powder||Na2HPO4, NaH2PO4, NaCL, Tween 20, Isinglass, et al.|
NOTE: * The working solutions should be clear.
*If working solution is prepared, please store at 2-8ºC, and use up within one week.
STORAGE AND PERIOD OF VALIDITY
It is valid for one year at room temperature. Please make solution fresh before the experiment.
Prepare a working solution by diluting the reagent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 120ml working solution.
1. Please avoid microbial contamination.
2. If precipitate has been found, please place the coating buffer in water bath with 37℃ till completely dissolution. Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.