ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP)
aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
- Product No.KSA153Po01
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.115ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|BioFactors||Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761|
|Food and Nutrition Sciences||Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684|
|Diabetes Research and Clinical Practice||Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520|
|Life Sciences||Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700|
|Annals of Hepatology||Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source|
|Biomedicine & Aging Pathology||Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348|
|European journal of pharmacology||Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413|
|Asian Pac J Cancer Prev.||Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529|
|Journal of Analytical Atomic Spectrometry||Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source|
|PLOS ONE||Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126|
|BMC Med Imaging||Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587|
|Tumour Biol||Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893|
|Acta Veterinaria Brno||The concentrations of selected blood serum proteins in calves during the first three months of life 85:33|
|Hepatobiliary Pancreat Dis Int||Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml|
|Journal of Applied Pharmaceutical Science||Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf|
|Frontiers in Physiology||Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats v04n07a07a|
|International Journal of Clinical and Experimental Medicine||Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model 1345-1349|
|Tumor Biology||Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway pubmed:28651490|
|Advances in Clinical and Experimental Medicine||Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles ISSN 2451-2680|
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|Sensors and Actuators B: Chemical||One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein 10.1016:j.snb.2018.03.038|
|Biomedicine & Pharmacotherapy||Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302|
|Journal of Investigative Surgery||Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation Pubmed:28107094|
|Asian Pacific Journal of Cancer Prevention||Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats Pubmed:29693337|
|Antioxid Redox Signal||The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma Pubmed:29687726|
|Cancer Biology||Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer|
|Cancer Biology||Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine|
|Biomedicine & Pharmacotherapy||The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932|
|Asian Journal of Pharmacy and Pharmacology||Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma|
|American Journal of Transplantation||Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence Pubmed: 31162867|
|Environ Toxicol Pharmacol.||Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311|
|Hepatology Research||Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385|
|REDOX REPORT||Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … Pubmed: 32172678|
|Scientific Reports||The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440|
|Nutr Cancer||Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation Pubmed: 32718197|
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|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA153Po01||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA153Po02||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA153Po03||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA153Po02||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|PAA153Po01||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|PAA153Po03||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|LAA153Po81||FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IF.|
|LAA153Po71||Biotin-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC.|
|SEA153Po||ELISA Kit for Alpha-Fetoprotein (AFP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEA153Po||Mini Samples ELISA Kit for Alpha-Fetoprotein (AFP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|LMA153Po||Magnetic Luminex Assay Kit for Alpha-Fetoprotein (AFP) ,etc.||Magnetic Luminex Assay for Antigen Detection.|
|KSA153Po01||ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP)||Main materials for "Do It (ELISA Kit) Yourself".|