Magnetic Luminex Assay Kit for Alpha-Fetoprotein (AFP) ,etc.
aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA153Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 1Plex2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
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This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (AFP) ,etc..
No significant cross-reactivity or interference between Alpha-Fetoprotein (AFP) ,etc. and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Alpha-Fetoprotein (AFP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Fetoprotein (AFP) ,etc. in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Fetoprotein (AFP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:AFP)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
|BioFactors||Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761|
|Food and Nutrition Sciences||Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684|
|Diabetes Research and Clinical Practice||Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520|
|Life Sciences||Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700|
|Annals of Hepatology||Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source|
|Biomedicine & Aging Pathology||Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348|
|European journal of pharmacology||Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413|
|Asian Pac J Cancer Prev.||Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529|
|Journal of Analytical Atomic Spectrometry||Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source|
|PLOS ONE||Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126|
|BMC Med Imaging||Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587|
|Tumour Biol||Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893|
|Acta Veterinaria Brno||The concentrations of selected blood serum proteins in calves during the first three months of life 85:33|
|Hepatobiliary Pancreat Dis Int||Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml|
|Journal of Applied Pharmaceutical Science||Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf|
|Frontiers in Physiology||Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats v04n07a07a|
|International Journal of Clinical and Experimental Medicine||Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model 1345-1349|
|Tumor Biology||Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway pubmed:28651490|
|Advances in Clinical and Experimental Medicine||Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles ISSN 2451-2680|
|Tumor biology||Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats pubmed:28720064|
|EBioMedicine||Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma Pubmed:30045829|
|Sensors and Actuators B: Chemical||One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein 10.1016:j.snb.2018.03.038|
|Biomedicine & Pharmacotherapy||Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302|
|Journal of Investigative Surgery||Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation Pubmed:28107094|
|Asian Pacific Journal of Cancer Prevention||Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats Pubmed:29693337|
|Antioxid Redox Signal||The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma Pubmed:29687726|
|Cancer Biology||Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer|
|Cancer Biology||Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine|
|Biomedicine & Pharmacotherapy||The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932|
|Asian Journal of Pharmacy and Pharmacology||Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma|
|American Journal of Transplantation||Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence Pubmed: 31162867|
|Environ Toxicol Pharmacol.||Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311|
|Hepatology Research||Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385|
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|Scientific Reports||The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440|
|Nutr Cancer||Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation Pubmed: 32718197|
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|Cytokine||QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023|
|Journal of Gastrointestinal Cancer||In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC 34698995|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA153Po01||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA153Po02||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA153Po03||Recombinant Alpha-Fetoprotein (AFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA153Po02||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|PAA153Po01||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|PAA153Po03||Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IP.|
|LAA153Po81||FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC; IF.|
|LAA153Po71||Biotin-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)||WB; IHC; ICC.|
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|LMA153Po||Magnetic Luminex Assay Kit for Alpha-Fetoprotein (AFP) ,etc.||Magnetic Luminex Assay for Antigen Detection.|
|KSA153Po01||ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP)||Main materials for "Do It (ELISA Kit) Yourself".|