≡

ELISA Kit for HLA-B Associated Transcript 3 (BAT3)

BAG6; Protein G3; Protein Scythe; BCL2-associated athanogene 6; BAG family molecular chaperone regulator 6; Large proline-rich protein BAG6

Specificity

This assay has high sensitivity and excellent specificity for detection of HLA-B Associated Transcript 3 (BAT3).
No significant cross-reactivity or interference between HLA-B Associated Transcript 3 (BAT3) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HLA-B Associated Transcript 3 (BAT3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPJ947Mu01 Recombinant HLA-B Associated Transcript 3 (BAT3) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ947Mu02 Recombinant HLA-B Associated Transcript 3 (BAT3) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ947Mu01 Polyclonal Antibody to HLA-B Associated Transcript 3 (BAT3) WB; IHC; ICC; IP.
PAJ947Mu02 Polyclonal Antibody to HLA-B Associated Transcript 3 (BAT3) WB; IHC; ICC; IP.
SEJ947Mu ELISA Kit for HLA-B Associated Transcript 3 (BAT3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ947Mu Multiplex Assay Kit for HLA-B Associated Transcript 3 (BAT3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.