ELISA Kit for Follistatin Like Protein 1 (FSTL1)

FRP; FSL1; Follistatin-related protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Follistatin Like Protein 1 (FSTL1).
No significant cross-reactivity or interference between Follistatin Like Protein 1 (FSTL1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Follistatin Like Protein 1 (FSTL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin Like Protein 1 (FSTL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-93 83
EDTA plasma(n=5) 85-104 92
heparin plasma(n=5) 79-94 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin Like Protein 1 (FSTL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 96-105% 79-95% 87-101%
EDTA plasma(n=5) 79-96% 88-95% 95-103% 78-92%
heparin plasma(n=5) 88-95% 92-101% 90-97% 83-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
The Journal of Rheumatology Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease. PubMed: 22660800
Archives of Physiology and Biochemistry Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells Pubmed: 23419164
Journal of Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation Pubmed:25118038
PLOS ONE Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary Bypass Pubmed:25171167
J Mol Med (Berl) Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance PubMed: 26199111
PLoS One. Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Men pmc:PMC4856269
JACC: Basic to Translational Science Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction. pubmed:27430031
Sci Rep Follistatin like-1 aggravates silica-induced mouse lung injury PMC5428474
International Journal of Clinical and Experimental Pathology Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortality ISSN:1936-2625
Endocrine Journal High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy state Pubmed: 30745500
PLoS One Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous … Pubmed: 31034503
British Journal of Cancer Improved early detection of ovarian cancer using longitudinal multimarker models Pubmed: 31937926
Cell Communication and Signaling Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in mice Pubmed: 32933544
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in rats Pubmed: 32412800
J Cardiol Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy 34334268
Int Heart J Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease 34853217
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