Multiplex Assay Kit for Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay)

FRP; FSL1; Follistatin-related protein 1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-99 96
EDTA plasma(n=5) 85-102 92
heparin plasma(n=5) 96-103 101
sodium citrate plasma(n=5) 84-102 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin Like Protein 1 (FSTL1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 98-105% 78-96% 79-91%
EDTA plasma(n=5) 81-89% 92-104% 78-103% 92-101%
heparin plasma(n=5) 80-98% 87-97% 95-102% 92-99%
sodium citrate plasma(n=5) 89-96% 79-98% 95-104% 78-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FSTL1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
The Journal of Rheumatology Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease. PubMed: 22660800
Archives of Physiology and Biochemistry Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells Pubmed: 23419164
Journal of Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation Pubmed:25118038
PLOS ONE Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary Bypass Pubmed:25171167
J Mol Med (Berl) Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance PubMed: 26199111
PLoS One. Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Men pmc:PMC4856269
JACC: Basic to Translational Science Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction. pubmed:27430031
Sci Rep Follistatin like-1 aggravates silica-induced mouse lung injury PMC5428474
International Journal of Clinical and Experimental Pathology Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortality ISSN:1936-2625
Endocrine Journal High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy state Pubmed: 30745500
PLoS One Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous … Pubmed: 31034503
British Journal of Cancer Improved early detection of ovarian cancer using longitudinal multimarker models Pubmed: 31937926
Cell Communication and Signaling Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in mice Pubmed: 32933544
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in rats Pubmed: 32412800
J Cardiol Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy 34334268
Int Heart J Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease 34853217
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