≡

ELISA Kit for Phosphofructokinase, Muscle (PFKM)

PFK1; PFKX; Phosphofructokinase 1; Phosphofructo-1-kinase isozyme A; 6-Phosphofructokinase Polypeptide X; Fructose-6-Phosphate 1-Phosphotransferase

Specificity

This assay has high sensitivity and excellent specificity for detection of Phosphofructokinase, Muscle (PFKM).
No significant cross-reactivity or interference between Phosphofructokinase, Muscle (PFKM) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cell Death & Disease The P2X7 receptor is a key modulator of aerobic glycolysis PubMed: 22898868
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD405Hu01 Recombinant Phosphofructokinase, Muscle (PFKM) Positive Control; Immunogen; SDS-PAGE; WB.
PAD405Hu01 Polyclonal Antibody to Phosphofructokinase, Muscle (PFKM) WB; IHC; ICC; IP.
MAD405Hu21 Monoclonal Antibody to Phosphofructokinase, Muscle (PFKM) WB; IHC; ICC; IP.
SED405Hu ELISA Kit for Phosphofructokinase, Muscle (PFKM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD405Hu Multiplex Assay Kit for Phosphofructokinase, Muscle (PFKM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.