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ELISA Kit for Ficolin 3 (FCN3)
FCNH; HAKA1; Collagen/Fibrinogen Domain Containing; Hakata Antigen; Collagen/fibrinogen domain-containing lectin 3 p35
- Product No.SEB903Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 504
For more details, please contact local distributors! US$ 720 US$ 3240 US$ 6120 US$ 50400
Specificity
This assay has high sensitivity and excellent specificity for detection of Ficolin 3 (FCN3).
No significant cross-reactivity or interference between Ficolin 3 (FCN3) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ficolin 3 (FCN3) and the recovery rates were calculated by comparing the measured value to the expected amount of Ficolin 3 (FCN3) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 87-101 | 94 |
EDTA plasma(n=5) | 95-104 | 98 |
heparin plasma(n=5) | 94-105 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 3 (FCN3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 3 (FCN3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ficolin 3 (FCN3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-105% | 92-99% | 94-104% | 92-103% |
EDTA plasma(n=5) | 82-93% | 96-105% | 90-97% | 96-103% |
heparin plasma(n=5) | 80-93% | 95-103% | 93-101% | 81-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
American Journal of Ophthalmology | Correlation between Ficolin-3 and Vascular Endothelial Growth Factor-to-Pigment Epithelium-Derived Factor Ratio in the Vitreous of Eyes with Proliferative Diabetic Retinopathy ScienceDirect: S0002939411004491 |
Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 5-D DIGE Pubmed: 23944848 |
Fundamental & Clinical Pharmacology | The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol Onlinelibrary: fcp.12074 |
The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402 |
Journal of Proteome Research | Targeted proteomics predict a sustained complete-response after transarterial chemoembolization and clinical outcomes in patients with hepatocellular carcinoma: a prospective cohort study pubmed:28112944 |
BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis Pubmed:29386009 |
ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis Pubmed: 32827456 |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
Immunol Lett | Ficolin-3 in Rheumatic Fever and Rheumatic Heart Disease 33232720 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
SEB903Mu | ELISA Kit for Ficolin 3 (FCN3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB903Mu | Multiplex Assay Kit for Ficolin 3 (FCN3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |