ELISA Kit for Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

Specificity

This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Pi (GSTp).
No significant cross-reactivity or interference between Glutathione S Transferase Pi (GSTp) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Glutathione S Transferase Pi (GSTp) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione S Transferase Pi (GSTp) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-93 89
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 83-95 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione S Transferase Pi (GSTp) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-92% 80-93% 96-103% 95-105%
EDTA plasma(n=5) 80-103% 90-97% 85-101% 85-102%
heparin plasma(n=5) 98-105% 94-102% 92-101% 81-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis Pubmed:27021019
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Arabian Journal of Chemistry Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
International Journal of Nature and Life Sciences The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands
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