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ELISA Kit for Podocin (PDCN)
NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant
- Product No.SEA938Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, urine, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.29ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
For more details, please contact local distributors! US$ 630 US$ 2835 US$ 5355 US$ 44100
Specificity
This assay has high sensitivity and excellent specificity for detection of Podocin (PDCN).
No significant cross-reactivity or interference between Podocin (PDCN) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Podocin (PDCN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Podocin (PDCN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788 |
Journal of Clinical Medicine | Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA938Hu01 | Recombinant Podocin (PDCN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA938Hu02 | Recombinant Podocin (PDCN) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA938Hu01 | Polyclonal Antibody to Podocin (PDCN) | WB; IHC; ICC; IP. |
LAA938Hu71 | Biotin-Linked Polyclonal Antibody to Podocin (PDCN) | WB; IHC; ICC. |
LAA938Hu81 | FITC-Linked Polyclonal Antibody to Podocin (PDCN) | WB; IHC; ICC; IF. |
MAA938Hu22 | Monoclonal Antibody to Podocin (PDCN) | WB |
MAA938Hu23 | Monoclonal Antibody to Podocin (PDCN) | WB |
MAA938Hu21 | Monoclonal Antibody to Podocin (PDCN) | WB; IHC; ICC; IP. |
SEA938Hu | ELISA Kit for Podocin (PDCN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA938Hu | ELISA Kit for Anti-Podocin Antibody (Anti-PDCN) | Enzyme-linked immunosorbent assay for Antibody Detection. |
LMA938Hu | Multiplex Assay Kit for Podocin (PDCN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA938Hu01 | ELISA Kit DIY Materials for Podocin (PDCN) | Main materials for "Do It (ELISA Kit) Yourself". |