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ELISA Kit for Cardiotrophin 1 (CT1)
CTF1
- Product No.SEA810Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.7pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
For more details, please contact local distributors! US$ 630 US$ 2835 US$ 5355 US$ 44100
Specificity
This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1).
No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 82-103 | 101 |
EDTA plasma(n=5) | 95-102 | 99 |
heparin plasma(n=5) | 85-99 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-88% | 81-92% | 96-104% | 78-104% |
EDTA plasma(n=5) | 78-97% | 93-105% | 78-97% | 84-92% |
heparin plasma(n=5) | 94-105% | 78-102% | 88-102% | 79-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
European Journal of Preventive Cardiology | Exercise rescued chronic kidney disease by attenuating cardiac hypertrophy through the cardiotrophin-1 -> LIFR/gp 130 -> JAK/STAT3 pathway. PubMed: 23064267 |
Journal of Hypertension | Is There Any Additional Cardiotrophin 1 Increase With Obesity in Hypertensive Patients?: Pp. 12.466 Lww: Source |
Journal of Hypertension | Cardiotrophyn 1 Levels in Nondipper Hypertensive Patients: Pp. 12.464 Lww: Source |
American Journal of Hypertension | Association Between Cardiotrophin 1 Levels and Central Blood Pressure in Untreated Patients With Essential Hypertension Pubmed: 24401751 |
Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153 |
Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599 |
Food & Function | Pterostilbene improves glycaemic control in rats fed an obesogenic diet: involvement of skeletal muscle and liver PubMed: 25998070 |
International journal of cardiology | Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535 |
Soldan sağa şantlı doğumsal kalp hastalıklı çocuklarda şant miktarı ile kardiyotrofin–1 ilişkisi Doi: 10.5505/deutfd.2018.25338.30074 | |
Annals of Nutrition and Metabolism | Effect of Salt Intake on the Serum Cardiotrophin-1 Levels in Chinese Adults Pubmed: 30408810 |
Atherosclerotic diseases | Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA810Hu01 | Recombinant Cardiotrophin 1 (CT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA810Hu01 | Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IP. |
LAA810Hu81 | FITC-Linked Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IF. |
LAA810Hu71 | Biotin-Linked Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC. |
MAA810Hu22 | Monoclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IP. |
SEA810Hu | ELISA Kit for Cardiotrophin 1 (CT1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA810Hu | Multiplex Assay Kit for Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |