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Multiplex Assay Kit for Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay)
CTF1
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA810Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 393
US$ 408
US$ 431
US$ 461
US$ 491
US$ 537
US$ 605
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 82-95 | 86 |
EDTA plasma(n=5) | 92-105 | 99 |
heparin plasma(n=5) | 87-102 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-90% | 97-105% | 97-105% | 91-98% |
EDTA plasma(n=5) | 82-104% | 93-105% | 78-89% | 95-104% |
heparin plasma(n=5) | 78-93% | 88-105% | 94-101% | 80-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:CT1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
European Journal of Preventive Cardiology | Exercise rescued chronic kidney disease by attenuating cardiac hypertrophy through the cardiotrophin-1 -> LIFR/gp 130 -> JAK/STAT3 pathway. PubMed: 23064267 |
Journal of Hypertension | Is There Any Additional Cardiotrophin 1 Increase With Obesity in Hypertensive Patients?: Pp. 12.466 Lww: Source |
Journal of Hypertension | Cardiotrophyn 1 Levels in Nondipper Hypertensive Patients: Pp. 12.464 Lww: Source |
American Journal of Hypertension | Association Between Cardiotrophin 1 Levels and Central Blood Pressure in Untreated Patients With Essential Hypertension Pubmed: 24401751 |
Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153 |
Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599 |
Food & Function | Pterostilbene improves glycaemic control in rats fed an obesogenic diet: involvement of skeletal muscle and liver PubMed: 25998070 |
International journal of cardiology | Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535 |
Soldan sağa şantlı doğumsal kalp hastalıklı çocuklarda şant miktarı ile kardiyotrofin–1 ilişkisi Doi: 10.5505/deutfd.2018.25338.30074 | |
Annals of Nutrition and Metabolism | Effect of Salt Intake on the Serum Cardiotrophin-1 Levels in Chinese Adults Pubmed: 30408810 |
Atherosclerotic diseases | Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA810Hu01 | Recombinant Cardiotrophin 1 (CT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA810Hu01 | Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IP. |
LAA810Hu81 | FITC-Linked Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IF. |
LAA810Hu71 | Biotin-Linked Polyclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC. |
MAA810Hu22 | Monoclonal Antibody to Cardiotrophin 1 (CT1) | WB; IHC; ICC; IP. |
SEA810Hu | ELISA Kit for Cardiotrophin 1 (CT1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA810Hu | Multiplex Assay Kit for Cardiotrophin 1 (CT1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |