ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10


This assay has high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10).
No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Interferon Gamma Induced Protein 10kDa (IP10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Gamma Induced Protein 10kDa (IP10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-101 90
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 87-97 91


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Gamma Induced Protein 10kDa (IP10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 88-97% 94-105% 86-97%
EDTA plasma(n=5) 92-99% 78-89% 78-105% 87-101%
heparin plasma(n=5) 90-101% 95-105% 84-98% 95-103%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
20 Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion. PubMed: 22288937
BMC Pregnancy and Childbirth Maternal circulating leukocytes display early chemotactic responsiveness during late gestation PubMed: PMC3561147
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
PLOS ONE The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart Transplant Pubmed:24798458
Front Pharmacol The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway PubMed: 26696888
Cytokine Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activation Pubmed:26891076
Journal of Experimental & Clinical Cancer Research LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. pubmed:28641579
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517
Hepatology Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice Pubmed: 31077413
Molecular Medicine Reports IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer Pubmed: 31173199
JOURNAL OF MOLECULAR NEUROSCIENCE Inflammatory Responses of Astrocytes Are Independent from Lipocalin 2 Pubmed: 32959226
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