≡

ELISA Kit for Pepsinogen C (PGC)

PG-C; Progastricsin; Gastricsin

Specificity

This assay has high sensitivity and excellent specificity for detection of Pepsinogen C (PGC).
No significant cross-reactivity or interference between Pepsinogen C (PGC) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pepsinogen C (PGC) and the recovery rates were calculated by comparing the measured value to the expected amount of Pepsinogen C (PGC) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 80-91 86
heparin plasma(n=5) 91-101 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsinogen C (PGC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsinogen C (PGC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsinogen C (PGC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-93% 90-104% 98-105% 94-101%
EDTA plasma(n=5) 81-94% 85-97% 80-104% 80-88%
heparin plasma(n=5) 90-98% 85-96% 94-102% 90-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Am J Physiol Cell Physiol Activation of dopamine D2 receptor promotes pepsinogen secretion by suppressing somatostatin release from the mouse gastric mucosa Pubmed:34986020
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA166Mu01 Recombinant Pepsinogen C (PGC) Positive Control; Immunogen; SDS-PAGE; WB.
RPA166Mu02 Recombinant Pepsinogen C (PGC) Positive Control; Immunogen; SDS-PAGE; WB.
PAA166Mu01 Polyclonal Antibody to Pepsinogen C (PGC) WB; IHC; ICC; IP.
PAA166Mu02 Polyclonal Antibody to Pepsinogen C (PGC) WB; IHC; ICC; IP.
SEA166Mu ELISA Kit for Pepsinogen C (PGC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA166Mu Multiplex Assay Kit for Pepsinogen C (PGC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CGA166Mu01 cDNA of Pepsinogen C (PGC) PCR; Protein Expression; etc.