Packages (Simulation)
Reagent Preparation
Image (I)
Image (II)
Certificate
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
Neurotrophin; Abrineurin
- Product No.SEA011Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.055ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 436
For more details, please contact local distributors! US$ 623 US$ 2804 US$ 5296 US$ 43610
Specificity
This assay has high sensitivity for detection of Brain Derived Neurotrophic Factor (BDNF).
100% cross-reactivity of Brain Derived Neurotrophic Factor (BDNF) was observed among Human, Mouse, Rat, Rabbit, Caprine, Equine.
Recovery
Matrices listed below were spiked with certain level of recombinant Brain Derived Neurotrophic Factor (BDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Derived Neurotrophic Factor (BDNF) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 95-103 | 99 |
EDTA plasma(n=5) | 88-102 | 95 |
heparin plasma(n=5) | 93-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Derived Neurotrophic Factor (BDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-104% | 78-89% | 90-99% | 87-94% |
EDTA plasma(n=5) | 82-96% | 90-101% | 94-103% | 96-104% |
heparin plasma(n=5) | 88-95% | 85-103% | 84-91% | 86-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
GIVEAWAYS
INCREMENT SERVICES
- Single-component Reagents of Assay Kit
- Lysis Buffer Specific for ELISA / CLIA
- Quality Control of Kit
- ELISA Kit Customized Service
- Disease Model Customized Service
- Serums Customized Service
- TGFB1 Activation Reagent
- Real Time PCR Experimental Service
- Streptavidin
- Fast blue Protein Stain solution
- Single-component Reagents of FLIA Kit
- Streptavidin-Agarose Beads
Magazine | Citations |
Toxicological Sciences | Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains PubMed: 21436126 |
Journal of the Neurological Sciences | Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219 |
International Conference on Bioscience | Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059 |
Neuroscience Letters | Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788 |
BioMed Research International | Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622 |
Behavioural Brain Research | The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810 |
Neurochemical Research | Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686 |
Exp Ther Med. | Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310 |
Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064 |
The International Journal af Applied Research in Veterinary Medicine | Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3 |
J Chem Neuroanat. | Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953 |
Int Immunopharmacol. | PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378 |
The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639 |
IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease |
Bipolar Disorders | Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449 |
Elsevier | Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article |
journal of allergy and clinical immunology | Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394 |
American Journal of Translational Research | A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979 |
European Journal of Pharmacology | Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206 |
Brain, Behavior, and Immunity | An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530 |
Stem Cell Research & Therapy | Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016 |
Pharmacological Research | N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442 |
Experimental Neurology | Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511 |
Nutrients | Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692 |
Neurotoxicity Research | Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048 |
Neurotoxicology | Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778 |
Neuroscience Letters | Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945 |
Applied Physiology Nutrition and Metabolism | Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists Pubmed: 30286300 |
Heliyon | Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195 |
BioMed Research International | Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling |
International Journal of Immunopathology and Pharmacology | Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799 |
American Journal of Emergency Medicine | Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke Pubmed: 30826210 |
Neurochemistry International | Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in mice Pubmed: 31077758 |
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA | Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression Pubmed: 30994834 |
Journal of Alzheimer's Disease | Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model Mice Pubmed: 31561359 |
Biological Trace Element Research | Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling Pubmed: 31797207 |
Psychopharmacology | Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats Pubmed: 32088835 |
Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Pubmed: 32010279 |
Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448 |
Journal of Clinical Neuroscience | CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children Pubmed: 32327377 |
MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … Pubmed: 32918393 |
Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084 |
COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease Pubmed: 32777662 |
Neurotoxicology | Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice Pubmed: 32522471 |
Journal of Veterinary Behavior | Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety |
Food & Function | Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial … |
Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine | |
bioinformatics and biomedical engineering | Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke |
Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi | |
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896 |
Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … 34239303 |
Einstein (Sao Paulo) | Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy 34586159 |
Metab Brain Dis | Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men 34762211 |
Transl Psychiatry | Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression 34824208 |
Iranian Journal of Basic Medical Sciences | Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons 34712429 |
J Sex Med | Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid … 34384732 |
Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway 34296452 |
J Endocrinol Invest | Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494 |
Clinical Rheumatology | Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by … Pubmed:35344113 |
Sci Rep | Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients Pubmed:35508633 |
Pharmaceutics | Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … Pubmed:35214053 |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
Journal of Complementary Medicine Research | Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder |
AFRICAN JOURNAL OF BIOCHEMISTRY RESEARCH | Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress |
Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA011Mi01 | Recombinant Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA011Mi01 | Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
MAA011Mi21 | Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
MAA011Mi26 | Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
LAA011Mi76 | Biotin-Linked Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC. |
SEA011Mi | ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA011Mi | Multiplex Assay Kit for Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA011Mi01 | Antibody Pair for Brain Derived Neurotrophic Factor (BDNF) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA011Mi01 | ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) | Main materials for "Do It (ELISA Kit) Yourself". |