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CLIA Kit for Toll Like Receptor 3 (TLR3)
CD283
- Product No.SCB989Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range5.49-4,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.93pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 605
For more details, please contact local distributors! US$ 864 US$ 3888 US$ 7344 US$ 60480
Specificity
This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 3 (TLR3).
No significant cross-reactivity or interference between Toll Like Receptor 3 (TLR3) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Toll Like Receptor 3 (TLR3) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 3 (TLR3) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-102 | 82 |
EDTA plasma(n=5) | 90-101 | 97 |
heparin plasma(n=5) | 89-96 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 3 (TLR3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 3 (TLR3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 3 (TLR3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 78-91% | 97-105% | 94-105% | 86-93% |
EDTA plasma(n=5) | 88-96% | 78-104% | 86-104% | 97-104% |
heparin plasma(n=5) | 83-101% | 83-97% | 95-104% | 87-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297 |
Heliyon | Daily oscillations in expression and responsiveness of Toll-like receptors in splenic immune cells Pubmed:29862343 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPB989Mu01 | Recombinant Toll Like Receptor 3 (TLR3) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB989Mu02 | Recombinant Toll Like Receptor 3 (TLR3) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB989Mu03 | Recombinant Toll Like Receptor 3 (TLR3) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB989Mu01 | Polyclonal Antibody to Toll Like Receptor 3 (TLR3) | WB; IHC; ICC; IP. |
PAB989Mu02 | Polyclonal Antibody to Toll Like Receptor 3 (TLR3) | WB; IHC; ICC; IP. |
PAB989Mu03 | Polyclonal Antibody to Toll Like Receptor 3 (TLR3) | WB; IHC; ICC; IP. |
LAB989Mu71 | Biotin-Linked Polyclonal Antibody to Toll Like Receptor 3 (TLR3) | WB; IHC; ICC. |
SEB989Mu | ELISA Kit for Toll Like Receptor 3 (TLR3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB989Mu | CLIA Kit for Toll Like Receptor 3 (TLR3) | Chemiluminescent immunoassay for Antigen Detection. |
LMB989Mu | Multiplex Assay Kit for Toll Like Receptor 3 (TLR3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSB989Mu01 | ELISA Kit DIY Materials for Toll Like Receptor 3 (TLR3) | Main materials for "Do It (ELISA Kit) Yourself". |