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CLIA Kit for Cartilage Oligomeric Matrix Protein (COMP)
MED; EDM1; EPD1; PSACH; THBS5; TSP-5; Pseudoachondroplasia,Epiphyseal Dysplasia 1,Multiple; Thrombospondin 5
- Product No.SCB197Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range1.37-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.49ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 638
For more details, please contact local distributors! US$ 912 US$ 4104 US$ 7752 US$ 63840
Specificity
This assay has high sensitivity and excellent specificity for detection of Cartilage Oligomeric Matrix Protein (COMP).
No significant cross-reactivity or interference between Cartilage Oligomeric Matrix Protein (COMP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cartilage Oligomeric Matrix Protein (COMP) and the recovery rates were calculated by comparing the measured value to the expected amount of Cartilage Oligomeric Matrix Protein (COMP) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 85-99 | 96 |
EDTA plasma(n=5) | 93-105 | 101 |
heparin plasma(n=5) | 94-102 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cartilage Oligomeric Matrix Protein (COMP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cartilage Oligomeric Matrix Protein (COMP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cartilage Oligomeric Matrix Protein (COMP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-93% | 83-92% | 98-105% | 99-105% |
EDTA plasma(n=5) | 78-105% | 78-104% | 94-101% | 94-104% |
heparin plasma(n=5) | 80-95% | 95-103% | 98-105% | 81-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Chinese Medical Journal | Genetic analysis and serum level of cartilage oligomeric matrix protein in patients with pseudoachondroplasia CMJ: 201082337960590 |
BioMed Research International | Decreased Plasma COMP and Increased Plasma CTX-II Levels in a Chinese Pseudoachondroplasia Family with Novel COMP Mutation pubmed:29104872 |
Molecular Medicine Reports | Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate‑induced osteoarthritis rats pubmed:29067461 |
Archives of Rheumatology | Cartilage Oligomeric Matrix Protein Levels in Synovial Fluid in Patients With Primary Knee Osteoarthritis And Healthy Controls: A Preliminary Comparative Analysis With Serum Cartilage Oligomeric Matrix Protein doi: 10.5606/ArchRheumatol.2017.6220 |
Genetic Testing and Molecular Biomarkers | Cartilage Oligomeric Matrix Protein Levels in Type 2 Diabetes Associated with Primary Knee Osteoarthritis Patients Pubmed: 30526057 |
International Journal of Clinical and Experimental Medicine | The relationship between the serum and synovial fluid osteopontin (OPN), cartilage oligomeric matrix protein (COMP), and the severity of knee osteoarthritis … |
British Journal of Medicine and Medical Research | Diagnostic Accuracy of Cartilage Oligomeric Matrix Protein (COMP), for Cartilage Damage in Rheumatoid Arthritis |