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Recombinant Complement Component 3a (C3a)

  • Product No.RPA387Rb01
  • Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass20.2kDa
  • Accurate Molecular Mass22kDa(Analysis of differences refer to the manual)
  • Residues & TagsCys581~Cys724 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point4.3
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 186  US$ 466  US$ 932  US$ 2796  US$ 6990 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
22 Immobilization of soluble complement receptor 1 on islets. ScienceDirect: S0142961211002596
Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845
Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587
SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977
Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247
Am J Reprod Immunol Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study PubMed: 26388133
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
Plos One Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice Pubmed:26872035
Digital Repository Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183
Colloids Surf B Biointerfaces. Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. pubmed:28131092
Pediatric Pulmonology Accuracy of complement activation product levels to detect infected pleural effusion in rats pubmed:28474458
Journal of Immunology The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression Pubmed:29367209
Artificial Cells Nanomedicine and Biotechnology Journal Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly … Pubmed:29873524
Cytotherapy Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation Doi: 10.1016/j.jcyt.2018.07.007
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
Clinical Immunology Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss
Materials Science and Engineering: C Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys
Nanomedicine Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion 33549818
EXPERIMENTAL EYE RESEARCH Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression 33493474
FASEB J C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
RPA387Rb01 Recombinant Complement Component 3a (C3a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA387Rb01 Polyclonal Antibody to Complement Component 3a (C3a) WB; IHC; ICC; IP.
SEA387Rb ELISA Kit for Complement Component 3a (C3a) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA387Rb Multiplex Assay Kit for Complement Component 3a (C3a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.