Packages (Simulation)

Polyclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)

Image (I)

DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30µg/mL Rabbit Anti-Human NDUFS1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 30µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cardiac Muscle Tissue;
Primary Ab: 20µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20µg/mL Rabbit Anti-Human NDUFS1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/mL Rabbit Anti-Human NDUFS1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 30µg/mL Rabbit Anti-Human NDUFS1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant NDUFS1, Human.
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: NDUFS1 knockout A549 cell lysate;
Predicted MW: 81,79,74,68kd
Observed MW: 74kd
Primary Ab: 2µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Human NDUFS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-NADH-Dehydrogenase-Ubiquinone-Fe-S-Protein-1-(NDUFS1)-PAJ794Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)

CI-75Kd; NADH-Coenzyme Q Reductase; NADH Dehydrogenase Iron-Sulfur Protein 1; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Product No.PAJ794Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPJ794Hu01-Recombinant NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NDUFS1. It has been selected for its ability to recognize NDUFS1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
	Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis
Nature Communications	Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPJ794Hu01	Recombinant NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAJ794Hu01	Polyclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)	WB,IHC
MAJ794Hu21	Monoclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)	WB; IHC; ICC; IP.
SEJ794Hu	ELISA Kit for NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ794Hu	Multiplex Assay Kit for NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.