Packages (Simulation)

Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)

Image (I)

DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Appendix Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human COTL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Thymus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human COTL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human COTL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure: FITC staining on IHC-P;
Sample: Hela cells.
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot ; Sample: Recombinant COTL1, Human.
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate; Lane3: Mouse Placenta lysate; Lane4: Mouse Kidney lysate
Primary Ab: 1µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: COTL1 knockout Hela cell lysate;
Predicted MW: 16kd
Observed MW: 16kd
Primary Ab: 1µg/ml Rabbit Anti-Human COTL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Coactosin-Like-Protein-1-(COTL1)-PAJ353Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)

CLP

Product No.PAJ353Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPJ353Hu01-Recombinant Coactosin Like Protein 1 (COTL1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COTL1. It has been selected for its ability to recognize COTL1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPJ353Hu01	Recombinant Coactosin Like Protein 1 (COTL1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAJ353Hu01	Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)	WB; IHC; ICC/IF
FAJ353Hu01	Anti-Coactosin Like Protein 1 (COTL1) Polyclonal Antibody	Flow cytometry.
MAJ353Hu21	Monoclonal Antibody to Coactosin Like Protein 1 (COTL1)	WB; IHC; ICC; IP.
SEJ353Hu	ELISA Kit for Coactosin Like Protein 1 (COTL1)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEJ353Hu	Mini Samples ELISA Kit for Coactosin Like Protein 1 (COTL1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ353Hu	Multiplex Assay Kit for Coactosin Like Protein 1 (COTL1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.