Packages (Simulation)

Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ITIH4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ITIH4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: U2OS cell
Primary Ab: 20µg/ml Rabbit Anti-Human ITIH4 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant ITIH4, Human.
Western Blot; Sample: Human Placenta lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human ITIH4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Serum;
Primary Ab: 1µg/ml Rabbit Anti-Human ITIH4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human ITIH4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Serum;
Primary Ab: 1µg/ml Rabbit Anti-Human ITIH4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Inter-Alpha-Globulin-Inhibitor-H4-(ITIH4)-PAH776Hu01.pdf

Certificate

Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)

IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein

Product No.PAH776Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPH776Hu01-Recombinant Inter Alpha-Globulin Inhibitor H4 (ITIH4)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.45mg/mL
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ITIH4. It has been selected for its ability to recognize ITIH4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Toxicology Letters	Biomarkers for methotrexate-induced liver injury: urinary protein profiling of psoriasis patients. Pubmed: 23830989
International Journal of Medical Microbiology	Proteomic demonstration of the recurrent presence of inter-alpha-inhibitor H4 heavy-chain during aspergillosis induced in an animal model Pubmed:24360996
Cancer Research and Treatment	Inter-alpha Inhibitor H4 as a Potential Biomarker Predicting the Treatment Outcomes in Patients with Hepatocellular Carcinoma pubmed:28724284
Diagnostics	Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848
Liver Cancer	Biomarkers for locally advanced hepatocellular carcinoma patients treated with liver-directed combined radiotherapy

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
APH776Hu01	Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Cell culture; Activity Assays.
APH776Hu03	Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Cell culture; Activity Assays.
RPH776Hu02	Recombinant Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH776Hu03	Recombinant Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH776Hu01	Recombinant Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAH776Hu03	Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)	WB; IHC; ICC; IP.
PAH776Hu02	Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)	WB; IHC; ICC; IP.
PAH776Hu01	Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)	WB; IHC; ICC; IP.
LAH776Hu71	Biotin-Linked Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)	WB; IHC; ICC.
MAH776Hu21	Monoclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)	WB; IHC; ICC; IP.
SEH776Hu	ELISA Kit for Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMH776Hu	Multiplex Assay Kit for Inter Alpha-Globulin Inhibitor H4 (ITIH4) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSH776Hu01	ELISA Kit DIY Materials for Inter Alpha-Globulin Inhibitor H4 (ITIH4)	Main materials for "Do It (ELISA Kit) Yourself".