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Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Stem Cells FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656
Diabetes. Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574
Journal of Cachexia, Sarcopenia and Muscle Muscle wasting and adipose tissue browning in infantile nephropathic cystinosis Doi: 10.1002
FASEB J Single-cell transcriptomics and functional target validation of brown adipocytes show their complex roles in metabolic homeostasis PubMed: 26304220
FASEB JOURNAL Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and … Pubmed: 32954574
Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis
Science reports All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice 33627760
Nature Communications Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF557Hu01 Recombinant Uncoupling Protein 1, Mitochondrial (UCP1) Positive Control; Immunogen; SDS-PAGE; WB.
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SEF557Hu ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF557Hu Multiplex Assay Kit for Uncoupling Protein 1, Mitochondrial (UCP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.