Packages (Simulation)

Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)

Image (I)

DAB staining on IHC-P; Sample: Rat Small intestine Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat ASAH2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat ASAH2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: Rat Small intestine lysate Primary Ab: 0.1µg/ml Rabbit Anti-Rat ASAH2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Small intestine lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Rat ASAH2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-N-Acylsphingosine-Amidohydrolase-2-(ASAH2)-PAE689Ra02.pdf

Certificate

Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)

HNAC1; non-lysosomal ceramidase; Neutral ceramidase

Product No.PAE689Ra02
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE689Ra03-Recombinant N-Acylsphingosine Amidohydrolase 2 (ASAH2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 114 266 US$ 266 US$ 380 US$ 950 US$ 3800
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ASAH2. It has been selected for its ability to recognize ASAH2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
scientific reports	New candidate blood biomarkers potentially associated with white matter hyperintensities progression 34253757

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPE689Ra03	Recombinant N-Acylsphingosine Amidohydrolase 2 (ASAH2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE689Ra02	Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)	WB; IHC; ICC; IP.
PAE689Ra03	Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)	WB; IHC; ICC; IP.
SEE689Ra	ELISA Kit for N-Acylsphingosine Amidohydrolase 2 (ASAH2)	Enzyme-linked immunosorbent assay for Antigen Detection.