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Polyclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ARNT. It has been selected for its ability to recognize ARNT in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Mol Neurobiol Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia PubMed: 26476840
The Role of Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor in Chronic Fetal Oxygen Deprivation
Sovremennye Tehnologii v Medicine РОЛЬ МОЗГОВОГО И ГЛИАЛЬНОГО НЕЙРОТРОФИЧЕСКИХ ФАКТОРОВ ПРИ ХРОНИЧЕСКОЙ ВНУТРИУТРОБНОЙ КИСЛОРОДНОЙ ДЕПРИВАЦИИ …
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