Packages (Simulation)

Polyclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)

Image (I)

DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

WB Image
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Mammary-Serine-Protease-Inhibitor--Maspin--PAD386Hu01.pdf

Certificate

Polyclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)

SERPINB5; PI5; Protease Inhibitor 5; Serpin Peptidase Inhibitor Clade B Member 5

Product No.PAD386Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD386Hu01-Recombinant Mammary Serine Protease Inhibitor (Maspin)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Maspin. It has been selected for its ability to recognize Maspin in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
International Journal of Cancer	Serpin B5 is a CEA-interacting biomarker for colorectal cancer Onlinelibrary: ijc.28494
Molecular and Cellular Biochemistry	Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway Pubmed:29363056

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD386Hu01	Recombinant Mammary Serine Protease Inhibitor (Maspin)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD386Hu01	Polyclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)	WB; IHC; ICC/IF
FAD386Hu01	Anti-Mammary Serine Protease Inhibitor (Maspin) Polyclonal Antibody	FCM
MAD386Hu21	Monoclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)	WB; IHC; ICC; IP.
SED386Hu	ELISA Kit for Mammary Serine Protease Inhibitor (Maspin)	Enzyme-linked immunosorbent assay for Antigen Detection.
CMD386Hu	Multiplex Assay Kit for Mammary Serine Protease Inhibitor (Maspin) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.
LMD386Hu	Multiplex Assay Kit for Mammary Serine Protease Inhibitor (Maspin) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.