Packages (Simulation)

Polyclonal Antibody to Hedgehog Homolog, Desert (DHH)

Image (I)

Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
AF488 staining on IF;
Sample: K562 cell
Primary Ab: 20µg/ml Rabbit Anti-Human DHH Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

WB Image
Western Blot; Sample: Mouse Cerebrum lysate
Primary Ab: 2μg/ml Rabbit Anti-Human DHH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Hedgehog-Homolog--Desert-(DHH)-PAD115Hu01.pdf

Certificate

Polyclonal Antibody to Hedgehog Homolog, Desert (DHH)

HHG3

Product No.PAD115Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD115Hu01-Recombinant Hedgehog Homolog, Desert (DHH)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DHH. It has been selected for its ability to recognize DHH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
The Journal of Biological Chemistry	Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signals PubMed: 22532569

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD115Hu02	Recombinant Hedgehog Homolog, Desert (DHH)	Positive Control; Immunogen; SDS-PAGE; WB.
APD115Hu01	Active Hedgehog Homolog, Desert (DHH)	Cell culture; Activity Assays.
RPD115Hu03	Recombinant Hedgehog Homolog, Desert (DHH)	Positive Control; Immunogen; SDS-PAGE; WB.
RPD115Hu01	Recombinant Hedgehog Homolog, Desert (DHH)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD115Hu01	Polyclonal Antibody to Hedgehog Homolog, Desert (DHH)	WB; IHC; ICC; IP.
PAD115Hu02	Polyclonal Antibody to Hedgehog Homolog, Desert (DHH)	WB; IHC; ICC; IP.
SED115Hu	ELISA Kit for Hedgehog Homolog, Desert (DHH)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD115Hu	Multiplex Assay Kit for Hedgehog Homolog, Desert (DHH) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.