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Polyclonal Antibody to Secreted Frizzled Related Protein 5 (SFRP5)

SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SFRP5. It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Diabetes Research and Clinical Practice Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance PubMed: 23290274
European Journal of Clinical Investigation Sfrp5 correlates with insulin resistance and oxidative stress PubMed: 23398169
Pediatric Research Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children Pubmed: 24603290
Clin Endocrinol (Oxf). SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children Pubmed: 24330025
The Journal of Clinical Endocrinology & Metabolism Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans Pubmed: 23185036
Atherosclerosis Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease Pubmed: 24530778
PLoS One. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation Pubmed:Pmc4072672
J Clin Endocrinol Metab. Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation Pubmed:24840810
Oncology Letters Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma Pubmed:25120720
Diabetes Metab Res Rev. Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls Pubmed:25139699
Clinical & Experimental Immunology The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis Pubmed:25382802
Diabetes Metab Res Rev Circulating SFRP5 levels are elevated in drug-na PubMed: 25139699
J Matern Fetal Neonatal Med Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels PubMed: 26100762
Eur J Clin Invest Sfrp5 associates with beta-cell function in humans Pubmed:27019073
Osteoporosis International Bone marrow sFRP5 level is negatively associated with bone formation markers pubmed:27986984
Diabetes/Metabolism Research and Reviews Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults pubmed:28303637
Cardiovascular Diabetology Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study pubmed:28851362
Arteriosclerosis, Thrombosis, and Vascular Biology  Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … Pubmed:29674475
Obesity Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376
J Bas Res Med Sci Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats article-1-352-en.pdf
Molecular Biology Reports The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome Pubmed: 30194556
Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling
British Journal Of Nutrition A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290
J Clin Endocrinol Metab Role of wnt5a in Metabolic Inflammation in Humans Pubmed: 30137542
Frontiers in Endocrinology Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients Pubmed: 32300333
CLINICAL BIOCHEMISTRY Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease Pubmed: 33188773
Sao Paulo Medical Journal Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … Pubmed: 32491086
Kidney Blood Press Res Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway 34469882
Cytokine Association of serum uromodulin with adipokines in dependence of type 2 diabetes 34920231
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470
JMIR Res Protoc Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition … Pubmed:35475790
Diabetology Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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SEC842Ra ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC842Ra Multiplex Assay Kit for Secreted Frizzled Related Protein 5 (SFRP5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.