Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NID Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NID Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant Nidogen, Human.
Western Blot; Sample: Human Lung lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human NID Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human NID Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Nidogen-(NID)-PAC466Hu01.pdf

Certificate

Polyclonal Antibody to Nidogen (NID)

Entactin

Product No.PAC466Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC466Hu01-Recombinant Nidogen (NID)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NID. It has been selected for its ability to recognize NID in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Clin Exp Metastasis.	Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression Pubmed:24807734
University of Oxford	Novel culture of human islets with pancreatic matrix proteins

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC466Hu01	Recombinant Nidogen (NID)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC466Hu01	Polyclonal Antibody to Nidogen (NID)	WB; IHC; ICC; IP.
MAC466Hu22	Monoclonal Antibody to Nidogen (NID)	WB; IHC; ICC; IP.
SEC466Hu	ELISA Kit for Nidogen (NID)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC466Hu	Multiplex Assay Kit for Nidogen (NID) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.