Polyclonal Antibody to Nucleolin (NCL)

C23; Protein C23

Product No.PAC242Hu03
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC242Hu03-Recombinant Nucleolin (NCL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
European Journal of Pharmaceutics and Biopharmaceutics	RNA G-quadruplex as supramolecular carrier for cancer-selective delivery
Mol Pharm	Drug Loading and Functional Efficacy of Cow, Buffalo, and Goat Milk-Derived Exosomes: A Comparative Study Pubmed:35195427
Frontiers in Physiology	Fine Tuning Inclusion in 2 of′-O-Methyl Phosphorothioate Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation Pubmed:34721051

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC242Hu01	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu02	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC242Hu03	Recombinant Nucleolin (NCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC242Hu02	Polyclonal Antibody to Nucleolin (NCL)	WB
PAC242Hu03	Polyclonal Antibody to Nucleolin (NCL)	WB
PAC242Hu01	Polyclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC/IF
MAC242Hu22	Monoclonal Antibody to Nucleolin (NCL)	WB; IHC; ICC; IP.
SEC242Hu	ELISA Kit for Nucleolin (NCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC242Hu	Multiplex Assay Kit for Nucleolin (NCL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
CMC242Hu	Multiplex Assay Kit for Nucleolin (NCL) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.