Packages (Simulation)

Polyclonal Antibody to Profilin 1 (PFN1)

Image (I)

DAB staining on IHC-P; Sample: Porcine Uterus Tissue Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PFN1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant PFN1, Human.
Western Blot; Sample: Human Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human PFN1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: LO2 cell lysate; Lane3: Rat Liver lysate; Lane4: Mouse Liver lysate; Lane5: Porcine Liver lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human PFN1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Profilin-1-(PFN1)-PAC233Hu01.pdf

Certificate

Polyclonal Antibody to Profilin 1 (PFN1)

Epididymis tissue protein Li 184a

Product No.PAC233Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC233Hu01-Recombinant Profilin 1 (PFN1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.43mg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PFN1. It has been selected for its ability to recognize PFN1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
PLoS One	Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680
Electrophoresis	Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosis Pubmed: 31081149
European Review for Medical and Pharmacological Sciences	Evaluation of profilin 1 as a biomarker in myocardial infarction Pubmed: 32767339
Clinical and Experimental Medicine	Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection
	ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ
	The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer
Biomed Pharmacother	Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status 34243097

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC233Hu01	Recombinant Profilin 1 (PFN1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC233Hu01	Polyclonal Antibody to Profilin 1 (PFN1)	WB
FAC233Hu11	Labelled Anti-Profilin 1 (PFN1) Polyclonal Antibody	FCM
MAC233Hu22	Monoclonal Antibody to Profilin 1 (PFN1)	WB; IHC; ICC; IP.
SEC233Hu	ELISA Kit for Profilin 1 (PFN1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC233Hu	Multiplex Assay Kit for Profilin 1 (PFN1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.