Packages (Simulation)

Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)

Image (I)

DAB staining on IHC-P; Sample: Mouse Uterus Tissue Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Raji cell
Primary Ab: 20µg/ml Rabbit Anti-Human MHCE Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant MHCE, Human.
Western Blot; Samples: Lane1: THP1 cell lysate; Lane2: Raji cell lysate; Lane3: Ramos cell lysate; Lane4: HUVEC cell lysate;
Primary Ab: 0.3µg/ml Rabbit Anti-Human MHCE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Major-Histocompatibility-Complex-Class-I-E-(MHCE)-PAB752Hu01.pdf

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Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)

HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E

Product No.PAB752Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB752Hu01-Recombinant Major Histocompatibility Complex Class I E (MHCE)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MHCE. It has been selected for its ability to recognize MHCE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Arthritis & Rheumatism	HLA–E gene polymorphism associated with susceptibility to kawasaki disease and formation of coronary artery aneurysms Asia: source
International Journal of Rheumatic Diseases	Soluble-HLA-E: A follow up biomarker in Takayasu arteritis, independent of HLA-E genotype pubmed:28425192
SCHIZOPHRENIA BULLETIN	Immune signatures of treatment resistant schizophrenia: a FACE-SZ study
Sci Rep	HLA-E circulating and genetic determinants in schizophrenia and bipolar disorder 34642395

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB752Hu01	Recombinant Major Histocompatibility Complex Class I E (MHCE)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB752Hu01	Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)	WB; IHC; ICC/IF
MAB752Hu22	Monoclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)	WB; IHC; ICC; IP.
SEB752Hu	ELISA Kit for Major Histocompatibility Complex Class I E (MHCE)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB752Hu	Multiplex Assay Kit for Major Histocompatibility Complex Class I E (MHCE) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.