Packages (Simulation)

Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)

Image (I)

DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

WB Image
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Skeletal muscle lysate
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: PFKP knockout A549 cell lysate;
Predicted MW: 85kd
Observed MW: 85kd
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Skeletal muscle lysate; Lane5: Hela cell lysate;
Primary Ab: 0.3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PFKP Polyclonal Antibody (Catalog PAB745Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Phosphofructokinase--Platelet-(PFKP)-PAB745Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)

PFK-C; PFKF; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; Phosphohexokinase

Product No.PAB745Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB745Hu01-Recombinant Phosphofructokinase, Platelet (PFKP)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PFKP. It has been selected for its ability to recognize PFKP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence: 5-20µg/mL;
Flow cytometry: 20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
J Cell Mol Med	Huaier shows anti©\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF©\1¦Á pathway 33377619

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB745Hu01	Recombinant Phosphofructokinase, Platelet (PFKP)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB745Hu01	Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)	WB; IHC; ICC/IF; FCM
MAB745Hu22	Monoclonal Antibody to Phosphofructokinase, Platelet (PFKP)	WB; IHC; ICC; IP.
SEB745Hu	ELISA Kit for Phosphofructokinase, Platelet (PFKP)	Enzyme-linked immunosorbent assay for Antigen Detection.
CMB745Hu	Multiplex Assay Kit for Phosphofructokinase, Platelet (PFKP) ,etc. by CBA (Cytometric Bead Array)	FLIA Kit for Antigen Detection.
LMB745Hu	Multiplex Assay Kit for Phosphofructokinase, Platelet (PFKP) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.