Packages (Simulation)

Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)

Image (I)

DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human LDHA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human LDHA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human LDHA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human LDHA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A549 cell
Primary Ab: 20µg/ml Rabbit Anti-Human LDHA Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant LDHA, Human.
Western Blot; Sample: Porcine Kidney lysate
Primary Ab: 0.15µg/ml Rabbit Anti-Human LDHA Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Testis lysate; Lane2: A431 cell lysate; Lane3: A375 cell lysate;
Primary Ab: 0.15µg/ml Rabbit Anti-Human LDHA Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Lactate-Dehydrogenase-A-(LDHA)-PAB370Hu01.pdf

Certificate

Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)

LDH-A; LDH-M; LDH1; PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59

Product No.PAB370Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB370Hu01-Recombinant Lactate Dehydrogenase A (LDHA)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LDHA. It has been selected for its ability to recognize LDHA in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
33	Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis pubmed:29248508
International Journal of Clinical and Experimental Medicine	Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease
	Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma
	Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC)
ACS Omega	SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics 34250342
Nutrients	Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383
Pharmaceutics	Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord … Pubmed:35214053

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB370Hu01	Recombinant Lactate Dehydrogenase A (LDHA)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB370Hu01	Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)	WB; IHC; ICC; IP.
LAB370Hu81	FITC-Linked Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)	WB; IHC; ICC; IF.
LAB370Hu71	Biotin-Linked Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)	WB; IHC; ICC.
MAB370Hu22	Monoclonal Antibody to Lactate Dehydrogenase A (LDHA)	WB; IHC; ICC; IP.
SEB370Hu	ELISA Kit for Lactate Dehydrogenase A (LDHA)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB370Hu	Multiplex Assay Kit for Lactate Dehydrogenase A (LDHA) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.