Packages (Simulation)

Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)

Image (I)

DAB staining on IHC-P; Samples: Human Lymphoma Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant CDK4, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: Rat Kidney lysate
Primary Ab: 1ug/ml Rabbit Anti-Human CDK4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: CDK4 knockout Hela cell lysate;
Predicted MW: 33kDa
Observed MW: 33kDa
Primary Ab: 1µg/ml Rabbit Anti-Human CDK4 Ab
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cyclin-Dependent-Kinase-4-(CDK4)-PAB233Hu01.pdf

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Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)

CMM3; PSK-J3; Cell division protein kinase 4

Product No.PAB233Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB233Hu01-Recombinant Cyclin Dependent Kinase 4 (CDK4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CDK4. It has been selected for its ability to recognize CDK4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB233Hu01	Recombinant Cyclin Dependent Kinase 4 (CDK4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB233Hu01	Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)	WB,IHC
MAB233Hu22	Monoclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)	WB; IHC; ICC; IP.
SEB233Hu	ELISA Kit for Cyclin Dependent Kinase 4 (CDK4)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB233Hu	Multiplex Assay Kit for Cyclin Dependent Kinase 4 (CDK4) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.