Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Small intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PLA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: 293T cell lysate Primary Ab: 0.2µg/ml Rabbit Anti-Human PLA1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: 293T cell lysate; Lane2: LO2 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human PLA1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PLA1 Polyclonal Antibody (Catalog PAM410Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Instruction Manual
Certificate
Polyclonal Antibody to Phospholipase A1 (PLA1)
PLA1A; ps-PLA1; NMD; Phosphatidylserine-Specific Phospholipase A1-Alpha
- Product No.PAB151Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB151Hu01-Recombinant Phospholipase A1 (PLA1)
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 100
US$ 232
US$ 332
US$ 830
US$ 3320
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PLA1. It has been selected for its ability to recognize PLA1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB151Hu02 | Recombinant Phospholipase A1 (PLA1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB151Hu01 | Recombinant Phospholipase A1 (PLA1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB151Hu01 | Polyclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC; IP; FCM |
| PAB151Hu02 | Polyclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC; IP; FCM |
| LAB151Hu71 | Biotin-Linked Polyclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC. |
| MAB151Hu22 | Monoclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC; IP. |
| LAB151Hu82 | FITC-Linked Monoclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC; IF. |
| LAB151Hu72 | Biotin-Linked Monoclonal Antibody to Phospholipase A1 (PLA1) | WB; IHC; ICC. |
| SEB151Hu | ELISA Kit for Phospholipase A1 (PLA1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB151Hu | Multiplex Assay Kit for Phospholipase A1 (PLA1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |