Packages (Simulation)

Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HaCaT cell
Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant PYGL, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: PYGL knockout HepG2 cell lysate;
Predicted MW: 97,93kd
Observed MW: 97kd
Primary Ab: 5µg/ml Rabbit Anti-Human PYGL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Glycogen-Phosphorylase--Liver-(PYGL)-PAA849Hu02.pdf

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KO-Validated

Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)

GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI

Product No.PAA849Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA849Hu02-Recombinant Glycogen Phosphorylase, Liver (PYGL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PYGL. It has been selected for its ability to recognize PYGL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-5µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
The University of Mustansiriyah	Biochemical and Immunohistochemical Study of Sildenafil on Various Tissues of Male Rats 4_2016_09_27!01_43_26_PM.pdf

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA849Hu01	Recombinant Glycogen Phosphorylase, Liver (PYGL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA849Hu02	Recombinant Glycogen Phosphorylase, Liver (PYGL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA849Hu02	Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)	WB,IHC
PAA849Hu01	Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)	WB; IHC; ICC; IP.
LAA849Hu71	Biotin-Linked Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)	WB; IHC; ICC.
MAA849Hu22	Monoclonal Antibody to Glycogen Phosphorylase, Liver (PYGL)	WB; IHC; ICC; IP.
SEA849Hu	ELISA Kit for Glycogen Phosphorylase, Liver (PYGL)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA849Hu	Multiplex Assay Kit for Glycogen Phosphorylase, Liver (PYGL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.