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Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI)

AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GPI. It has been selected for its ability to recognize GPI in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oncology Reports Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor PubMed: 23027359
Arthritis & Rheumatology Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritis Pubmed:26945549
Rheumatoid Arthritis Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritis pubmed:26945549
Infection and Immunity The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria Pubmed:29784863
Journal of Proteomics Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility Pubmed: 31394311
Scientific Reports The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI … Pubmed: 31959860
JOURNAL OF IMMUNOLOGY T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein Pubmed: 33199538
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