- KO-Validated
Polyclonal Antibody to Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- Product No.PAA537Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA537Ra01-Recombinant Enolase, Neuron Specific (NSE)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 103
For more details, please contact local distributors! US$ 239 US$ 342 US$ 855 US$ 3420
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- Antibody Labeling Customized Service
- Protein A/G Purification Column
- Staining Solution for Cells and Tissue
- Positive Control for Antibody
- Tissue/Sections Customized Service
- Phosphorylated Antibody Customized Service
- Western Blot (WB) Experiment Service
- Immunohistochemistry (IHC) Experiment Service
- Immunocytochemistry (ICC) Experiment Service
- Flow Cytometry (FCM) Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Immunofluorescence (IF) Experiment Service
- Buffer
- DAB Chromogen Kit
- SABC Kit
- Long-arm Biotin Labeling Kit
- Real Time PCR Experimental Service
Magazine | Citations |
Journal of Neurotrauma | The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats Liebert: 20091210 |
20 | Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder Wiley: source |
Journal of Pharmacology and Toxicology | Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice Medwell: 2011679690 |
Addiction Biology | Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence PubMed: 21995595 |
Placenta | Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia Pubmed: 24144948 |
J Ethnopharmacol. | Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. Pubmed: 24291152 |
J Neurosurg. | Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. Pubmed: 23540266 |
Analytical Methods | A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out Rsc: Source |
Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits Pubmed:25111247 |
Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns Pubmed:25358349 |
Neuroscience Letters | Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. Pubmed:25562631 |
中华临床医师杂志 | 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 Journal: Fulltext |
Am J Trop Med Hyg | Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos PubMed: 26055741 |
Neurosci Lett | Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats PubMed: 25562631 |
Physiol Rep | S100B and NSE serum concentrations after simulated diving in rats PubMed: 26462746 |
Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions PubMed: 26184156 |
PLoS One | Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits PubMed: 26645542 |
Фармакология, клиническая фармакология | Волгоградский государственный медицинский университет uploads:dsovet |
Analytical Methods | Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction articlelanding:c5ay03174e |
中国临床药理学与治疗学 | 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 article:9794 |
Asian Pacific Journal of Tropical Medicine | Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955 |
Izvorni znanstveni članak | SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI 262238 |
Neurourol Urodyn | Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid pubmed:27490041 |
Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830 |
cellular physiology and biochemistry | Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … pubmed:29040978 |
Brain Research | Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway Pubmed:29510144 |
Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336 |
Journal of the Neurological Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … Pubmed:29801873 |
Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper Doi: 10.22099/IJVR.2018.4943 |
Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway |
The Eurasian Journal of Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema |
Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … |
ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … Pubmed: 32214270 |
Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366 |
European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … Pubmed: 32432773 |
Neurobiology of Learning and Memory | Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats |
The role of light desynchronosis in the development of stress-induced aging | |
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ … | |
Chinese Journal of Analytical Chemistry | Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis |
Ther Adv Chronic Dis | The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial 34262678 |
J Surg Res | Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway 34399353 |
Cephalalgia | Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome 34644195 |