Packages (Simulation)

Polyclonal Antibody to Cytokeratin 1 (CK1)

Image (I)

DAB staining on IHC-P; Sample: Porcine Skin Tissue Primary Ab: 10µg/ml Rabbit Anti-Rat CK1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: Rat Skin lysate Primary Ab: 0.05µg/ml Rabbit Anti-Rat CK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Rat Colon lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat KRT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat KRT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cytokeratin-1-(CK1)-PAA492Ra01.pdf

Certificate

Polyclonal Antibody to Cytokeratin 1 (CK1)

KRT1; EHK1; K1; KRT1A; KRT1-A; Keratin 1; Epidermolytic Hyperkeratosis; Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Type-II keratin Kb1

Product No.PAA492Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA492Ra02-Recombinant Cytokeratin 1 (CK1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 103 239 US$ 239 US$ 342 US$ 855 US$ 3420
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CK1. It has been selected for its ability to recognize CK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPA492Ra01	Recombinant Cytokeratin 1 (CK1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA492Ra02	Recombinant Cytokeratin 1 (CK1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA492Ra01	Polyclonal Antibody to Cytokeratin 1 (CK1)	WB; IHC; ICC; IP.
PAA492Ra02	Polyclonal Antibody to Cytokeratin 1 (CK1)	WB; IHC; ICC; IP.
LAA492Ra71	Biotin-Linked Polyclonal Antibody to Cytokeratin 1 (CK1)	WB; IHC; ICC.
SEA492Ra	ELISA Kit for Cytokeratin 1 (CK1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA492Ra	Multiplex Assay Kit for Cytokeratin 1 (CK1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.