Packages (Simulation)
Image (I)
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AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human COL4a5 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Instruction Manual
Certificate
Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
COL4-A5; ASLN; ATS; CA54; Collagen Alpha-5(IV)chain; Alport Syndrome
- Product No.MAC141Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)E3
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPC141Hu01-Recombinant Collagen Type IV Alpha 5 (COL4a5)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 107
US$ 249
US$ 355
US$ 888
US$ 3550
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against COL4a5. It has been selected for its ability to recognize COL4a5 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Experimental and Molecular Pathology | Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909 |
| American Journal of Respiratory Cell and Molecular Biology | Basement Membrane Remodelling Controls Endothelial Function in IPAH Pubmed: 32160015 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC141Hu01 | Recombinant Collagen Type IV Alpha 5 (COL4a5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC141Hu01 | Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB;ICC/IF |
| LAC141Hu71 | Biotin-Linked Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC. |
| MAC141Hu21 | Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IP. |
| MAC141Hu22 | Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IP. |
| LAC141Hu82 | FITC-Linked Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IF. |
| SEC141Hu | ELISA Kit for Collagen Type IV Alpha 5 (COL4a5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC141Hu | Multiplex Assay Kit for Collagen Type IV Alpha 5 (COL4a5) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |