Packages (Simulation)
Image (II)
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Western Blot; Samples: Lane1: Rat Stomach lysate; Lane2: Rat Placenta lysate;
Primary Ab: 0.1µg/ml Mouse Anti-Human IL35 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: THP1 cell lysate
Primary Ab: 0.3µg/ml Mouse Anti-Human IL35 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Instruction Manual
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Monoclonal Antibody to Interleukin 35 (IL35)
- Product No.MAC008Hu27
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C5
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPC008Hu01-Recombinant Interleukin 35 (IL35)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 113
US$ 263
US$ 376
US$ 940
US$ 3760
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against IL35. It has been selected for its ability to recognize IL35 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
| Magazine | Citations |
| Oncology Letters | Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403 |
| Journal of Maternal-Fetal and Neonatal Medicine | Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856 |
| Journal of Reproductive Immunology | What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037 |
| Human Immunology | The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776 |
| Folia Biologica (Praha) | Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110 |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532 |
| Rom J Morphol Embryol. | The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266 |
| Journal of Immunotherapy | Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106 |
| PLOS ONE | Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444 |
| Rheumatology (Oxford) | Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346 |
| Cytokine | Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938 |
| J Biol Chem | Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094 |
| Br J Haematol | Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666 |
| Autoimmunity Reviews | New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383 |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19 |
| Clinical Rheumatology | The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600 |
| Respiratory Medicine | Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538 |
| International Journal of Molecular Medicine | Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435 |
| 上海口腔医学 | IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析 QK:98604X |
| Reproductive Sciences | Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation pubmed:28659009 |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis issn:2542-3975 |
| The Journal of Rheumatology | Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis pubmed:29247146 |
| Cellular & Molecular Immunology | Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro pubmed:28713164 |
| Molecular Medicine Reports | Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310 |
| Asian pacific journal of allergy and immunology | Evaluation of serum interleukin- 35 level in children with persistent asthma. pubmed:27996283 |
| PLoS One | Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio pubmed:29236782 |
| Hepatology Communications | Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation 10.1002/hep4.1052 |
| International Journal of Clinical and Experimental Medicine | Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901 |
| OncoTargets and Therapy | Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. pubmed:28761357 |
| Annals of Rheumatic Diseases | AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features 10.1136:annrheumdis-2018-eular.2605 |
| Tohoku Journal of Experimental Medicine | Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients Pubmed:29576585 |
| Turkish Journal Of Medical Sciences | The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245 |
| Oncotarget | Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas Pubmed:29721156 |
| Journal of Clinical Rheumatology | Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features. Pubmed: 30431486 |
| Oncology Letters | Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer |
| Journal of Clinical Investigation | Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35 Pubmed: 31264967 |
| Cells | CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired Pubmed: 32111105 |
| Cytokine | Interleukin-35 in idiopathic inflammatory myopathies Pubmed: 33128920 |
| FEBS Open Bio | IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 Pubmed: 33064893 |
| Cancer Cell International | Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity Pubmed: 33041668 |
| Journal of Neuroinflammation | Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain Pubmed: 32375811 |
| Int Immunopharmacol | Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis 33307515 |
| Archives of Medical Science | Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab) 33488880 |
| Asia Pac Allergy | Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis 34386410 |
| Int Immunopharmacol | Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expression Pubmed:35247859 |
| Biology and Medical Researches | Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city |
| Research Square | Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC008Hu01 | Recombinant Interleukin 35 (IL35) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC008Hu01 | Active Interleukin 35 (IL35) | Cell culture; Activity Assays. |
| PAC008Hu01 | Polyclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu25 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu22 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu23 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu24 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu29 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu21 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu28 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu26 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| MAC008Hu27 | Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC; IP. |
| LAC008Hu72 | Biotin-Linked Monoclonal Antibody to Interleukin 35 (IL35) | WB; IHC; ICC. |
| SEC008Hu | ELISA Kit for Interleukin 35 (IL35) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEC008Hu | High Sensitive ELISA Kit for Interleukin 35 (IL35) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC008Hu | Multiplex Assay Kit for Interleukin 35 (IL35) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| PSC008Hu01 | Antibody Pair for Interleukin 35 (IL35) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSC008Hu01 | ELISA Kit DIY Materials for Interleukin 35 (IL35) | Main materials for "Do It (ELISA Kit) Yourself". |