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Western Blot; Sample: Human Lung lysate; Primary Ab: 3µg/ml Mouse Anti-Human VCL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Rat Heart lysate;
Primary Ab: 3µg/ml Mouse Anti-Human VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 3µg/ml Mouse Anti-Human VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

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Instruction Manual

Monoclonal-Antibody-to-Vinculin-(VCL)-MAB839Hu22.pdf

Certificate

Monoclonal Antibody to Vinculin (VCL)

MVCL; Metavinculin

Product No.MAB839Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)C3
Ig Isotype IgG2a Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB839Hu01-Recombinant Vinculin (VCL)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration1mg/mL
Organism Species MoreRattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 125 293 US$ 293 US$ 418 US$ 1045 US$ 4180
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
PBMCs S-NO proteomic signature in heart failure	S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure journals:1384523
J Cell Sci	PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals Pubmed:35044463

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB839Hu01	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB839Hu02	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB839Hu01	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
PAB839Hu02	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
CAB839Hu01	Anti-Vinculin (VCL) Polyclonal Antibody	Loading Control of WB.
LAB839Hu71	Biotin-Linked Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC.
MAB839Hu22	Monoclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
MAB839Hu21	Monoclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
CAB839Hu22	Anti-Vinculin (VCL) Monoclonal Antibody	Loading Control of WB
SEB839Hu	ELISA Kit for Vinculin (VCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEB839Hu	ELISA Kit for Anti-Vinculin Antibody (Anti-VCL)	Enzyme-linked immunosorbent assay for Antibody Detection.
LMB839Hu	Multiplex Assay Kit for Vinculin (VCL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.