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Monoclonal Antibody to Defensin Beta 1 (DEFb1)

B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1

  • Product No.MAB373Po21
  • Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 126  US$ 294  US$ 420  US$ 1050  US$ 4200 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against DEFb1. It has been selected for its ability to recognize DEFb1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Appl Environ Microbiol. Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source
British Journal of Nutrition Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699
International Journal of Dairy Technology Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111
Molecular Nutrition Food Research Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948
Frontiers in Microbiology Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590
International Immunopharmacology Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347
cyberleninka EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Animals Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218
Sci Rep Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937
J Mol Histol Isolation, culture, and identification of ceruminous gland cells Pubmed:35113280
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPB373Po01 Recombinant Defensin Beta 1 (DEFb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB373Po01 Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
LAB373Po81 FITC-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IF.
LAB373Po71 Biotin-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC.
MAB373Po21 Monoclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
SEB373Po ELISA Kit for Defensin Beta 1 (DEFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB373Po Multiplex Assay Kit for Defensin Beta 1 (DEFb1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.