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Western Blot; Sample: Human Leukocyte lysate Primary Ab: 0.4µg/ml Mouse Anti-Human CD8a Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Monoclonal Antibody to Cluster Of Differentiation 8a (CD8a)
p32; CD8-A; Leu2; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
- Product No.MAB099Hu25
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)D4
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen EPB099Hu61-Eukaryotic Cluster Of Differentiation 8a (CD8a)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CD8a. It has been selected for its ability to recognize CD8a in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494 |