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Monoclonal Antibody to Estrogen Receptor Alpha (ERa)

ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1

  • Product No.MAB050Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 115  US$ 267  US$ 382  US$ 955  US$ 3820 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against ERa. It has been selected for its ability to recognize ERa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829
Biochem Biophys Res Commun Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924
cellular microbiology Influenza A virus NS1 protein-induced JNK activation and apoptosis are not functionally linked. pubmed:28076660
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Cellular Physiology and Biochemistry Antiestrogenic Activity of the Xi-Huang Formula for Breast Cancer by Targeting the Estrogen Receptor α Pubmed:29975948
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
Clinical Breast Cancer Vitamin D receptor in breast cancer tissues and its relation to estrogen receptor alpha (ER-a) gene expression and serum 25-hydroxyvitamin D levels in Egyptian  Doi: 10.1016/j.clbc.2018.12.019
Journal of geriatric psychiatry and neurology ERα Gene Promoter Methylation in Cognitive Function and Quality of Life of Patients With Alzheimer Disease Pubmed: 30947592
mBio The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription … 34488451
Cardiometry Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB050Ra01 Recombinant Estrogen Receptor Alpha (ERa) Positive Control; Immunogen; SDS-PAGE; WB.
PAB050Ra01 Polyclonal Antibody to Estrogen Receptor Alpha (ERa) WB; IHC; ICC; IP.
MAB050Ra21 Monoclonal Antibody to Estrogen Receptor Alpha (ERa) WB; IHC; ICC; IP.
SEB050Ra ELISA Kit for Estrogen Receptor Alpha (ERa) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB050Ra Multiplex Assay Kit for Estrogen Receptor Alpha (ERa) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.