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Monoclonal Antibody to Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

  • Product No.MAA471Ca21
  • Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 114  US$ 266  US$ 380  US$ 950  US$ 3800 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against OC. It has been selected for its ability to recognize OC in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Asian-Australasian Association of Animal Societies Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter? Ajas: source
Nutrition Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717
Experimental Biology and Medicine Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured mice Rsm: source
Archives of Oral Biology Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats ScienceDirect: S0003996912001021
22 Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces ScienceDirect: S0142961212000865
Life Sciences Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes PubMed: 23357248
Romanian Journal of Morphology and Embryology Serum changes induced by intramedullar experimental administration of bisphosphonates PubMed: 21424089
International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity PubMed: 23452891
Journal of Physiology and Pharmacology Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone PubMed: 23211309
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
Chinese Medicine Puerarin promotes osteogenesis and inhibits adipogenesis in vitro PubMed: PMC3765709
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells PubMed: PMC3611891
J Anim Physiol Anim Nutr (Berl). Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancy Pubmed: 22716040
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International Journal of Cardiology Valvular osteoclasts in calcification and aortic valve stenosis severity. Pubmed: 23452891
Food Science & Nutrition Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 Onlinelibrary: fsn3.109
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Tissue Eng Part A. Toward the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone Substitute Pubmed:25761235
Basic & Clinical Pharmacology & Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source
Food Sci Nutr. Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 Pubmed:Pmc4221832
Journal of Cranio-Maxillofacial Surgery The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed:24485270
Naunyn-Schmiedeberg's Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
American Diabetes Association Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopenia Pubmed:25633418
International Journal of Nanomedicine Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation Pubmed:25678785
Medical Journal fo the Islamic Republic of Iran Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models Pubmed:25679008
BioMed Research International Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathway Pubmed:25834823
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Int J Mol Med Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway PubMed: 26677102
Experimental And Therapeutic Medicine Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway Pubmed:27347050
International Journal of Life Sciences Research Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats profile:303311214
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Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
J Bone Miner Metab MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967
Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats :
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561
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Applied Surface Science The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption S0169433216326861
Nutrition. Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549
Chemico-Biological Interactions Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway pubmed:27387537
Biomedicine & Pharmacotherapy Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective … S0753332216308484
Experimental and Therapeutic Medicine Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits. pubmed:28105092
Institute of Pharmacology Effects of long-term administration of pantoprazole on bone mineral density in young male rats. pubmed:27552060
Indian Journal of Orthopaedics N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits. pubmed:27512225
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Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X
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Materials Science & Engineering C-Materials for Biological Applications BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model Pubmed:30033316
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Life Sciences Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway Pubmed:29883721
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
Royal Society of Chemistry Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances c7tb02620j
Molecular Medicine Reports Effects of constrained dynamic loading, CKIP‑1 gene knockout and combination stimulations on bone loss caused by mechanical unloading Pubmed:29956799
Journal of Ultrasound in Medicine A Comparison of 1‐and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study Pubmed:29781183
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American Scientific Publishers Effects of KLD-12/Recombinant Human Bone Morphogenetic Protein-2 Nanofiber Scaffold on the Osteogenic Activity of Rabbit Bone Marrow Mesenchymal Stem Cells 10.1166:jbt.2018.1719
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International Journal of Clinical and Experimental Medicine Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats
Nanoscale Beta-Cyclodextrin Modified Mesoporous Bioactive Glass Nanoparticles/Silk Fibroin Hybrid Nanofibers as an Implantable Estradiol Delivery System for the Potential … Pubmed: 30255905
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Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771
Tropical Journal of Pharmaceutical Research Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway
Bone & Joint Research Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
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ACS Applied Materials & Interfaces Osteoinductivity of porous biphasic calcium phosphate ceramic spheres with nanocrystalline and their efficacy in guiding bone regeneration Pubmed: 30629405
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization Pubmed: 30689288
Chemico-Biological Interactions Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080
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Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809
American Journal of Translational Research Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
Journal of Orthopaedic Surgery and Research Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
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Tropical Journal of Pharmaceutical Research Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.
medical science monitor Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132 … Pubmed: 30923307
Toxicology and applied pharmacology Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR/S6K1/S6 signaling pathways Pubmed: 31051157
Journal of Innovations in Pharmaceutical and Biological Sciences Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A A dual functional bone‐defect‐filling material with sequential antibacterial and osteoinductive properties for infected bone defect repair Pubmed: 31173657
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International Journal of Nanomedicine Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway Pubmed: 31632013
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209
International Journal of Environmental Research and Public Health Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats Pubmed: 31739465
Pharmacology MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal Pubmed: 31694033
Brazilian Journal of Medical and Biological Research Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals Pubmed: 31826180
Biomedicine & Pharmacotherapy Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone loss Pubmed: 31846839
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells Pubmed: 32064734
CELL BIOCHEMISTRY AND FUNCTION HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinase Pubmed: 32567086
Cell Death & Disease QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism Pubmed: 32382069
Molecular Medicine Reports Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism Pubmed: 33000198
Food & Function Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the … Pubmed: 33140795
ADVANCED FUNCTIONAL MATERIALS A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration
Revista Argentina de Clínica Psicológica Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene
淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
Chemical Engineering Journal Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances
Surfaces and Interfaces In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching
J Bone Miner Res Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway 33950533
Biochemical Engineering Journal Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis 34233019
PHARMACEUTICAL BIOLOGY Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043
Evid Based Complement Alternat Med Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780
ACS Appl Mater Interfaces Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic Nanomedicine Pubmed:35311248
Molecules Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells Pubmed:35684342
Journal of Exercise Science & Fitness Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young Females Pubmed:35096081
Journal of Bionic Engineering Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA471Ca01 Recombinant Osteocalcin (OC) Positive Control; Immunogen; SDS-PAGE; WB.
PAA471Ca01 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Ca21 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
SEA471Ca ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA471Ca Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.