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Multiplex Assay Kit for Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay)
SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)
(Note: Up to 8-plex in one testing reaction)
- Product No.LMC130Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 449
US$ 467
US$ 492
US$ 527
US$ 562
US$ 613
US$ 691
Result
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Specificity
This assay has high sensitivity for detection of Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay).
100% cross-reactivity of Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) was observed among Human, Mouse, Rat.
Recovery
Matrices listed below were spiked with certain level of recombinant Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-92 | 88 |
EDTA plasma(n=5) | 91-99 | 94 |
heparin plasma(n=5) | 88-105 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-90% | 91-101% | 92-104% | 81-101% |
EDTA plasma(n=5) | 79-101% | 96-103% | 79-104% | 80-88% |
heparin plasma(n=5) | 81-90% | 94-101% | 79-95% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:NOG) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Arthritis Rheumatol | Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis PubMed: 26413886 |
Arthritis Rheumatol. | Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis Pubmed:26413886 |
Clin Exp Pharmacol Physiol. | Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways. Pubmed:26385023 |
European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. pubmed:28266688 |
Arthritis & Rheumatology | mbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal OsteogenicDifferentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis. pubmed:26413886 |
Biotechnology and Applied Biochemistry | The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells. pubmed:28981962 |
Heliyon | Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis pubmed:29167826 |
Journal of Cellular Biochemistry | Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular … Pubmed: 31006911 |
Aging Male | Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment Pubmed: 32160825 |
J Reconstr Microsurg | Effect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model Pubmed: 31995818 |
Biochem Biophys Rep | Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP Pubmed:35005256 |
Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
SEC130Mi | ELISA Kit for Noggin (NOG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEC130Mi | High Sensitive ELISA Kit for Noggin (NOG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC130Mi | Multiplex Assay Kit for Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |