Multiplex Assay Kit for Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay)

RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-101 95
EDTA plasma(n=5) 84-98 89
heparin plasma(n=5) 97-105 101
sodium citrate plasma(n=5) 80-99 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Reverse Triiodothyronine (rT3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 82-96% 96-105% 99-105%
EDTA plasma(n=5) 87-96% 89-103% 80-89% 89-104%
heparin plasma(n=5) 91-105% 89-103% 80-94% 82-96%
sodium citrate plasma(n=5) 78-102% 78-91% 81-101% 87-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:rT3) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
J Clin Endocrinol Metab. N-Acetylcysteine Administration Prevents Nonthyroidal Illness Syndrome in Patients With Acute Myocardial Infarction: A Randomized Clinical Trial Pubmed:25148231
Tierärztliche Hochschule Hannover Untersuchungen zum Schilddrüsenstoffwechsel der Milchkuh in Abhängigkeit des Laktations-, Reproduktions- und Gesundheitsstatus dissertations:steinhoffl_ws16.pdf
Glia. Inflammation severely alters thyroid hormone signaling in the central nervous system duringexperimental allergic encephalomyelitis in rat: Direct impact on OPCs differentiation failure. pubmed:27404574
Scientific Reports A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients Pubmed: 31519956
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