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Multiplex Assay Kit for Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 96
EDTA plasma(n=5) 78-101 88
heparin plasma(n=5) 91-105 101
sodium citrate plasma(n=5) 82-92 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 80-96% 97-105% 92-99%
EDTA plasma(n=5) 92-101% 80-94% 96-103% 79-97%
heparin plasma(n=5) 78-105% 79-88% 83-104% 90-101%
sodium citrate plasma(n=5) 80-104% 93-103% 83-101% 91-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:MASP1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Pathology Increased Circulating Plasma Mannose Binding Lectin Associated Serine Protease (Masp) As A Result of Enu-Induced Mutation in Masp-1 Lww: Source
The Journal fo Biological Chemistry Bisretinoid-mediated Complement Activation on Retinal Pigment Epithelial Cells Is Dependent on Complement Factor H Haplotype Pubmed:24550392
the journal of immunology The Levels of the Lectin Pathway Serine Protease MASP-1 and Its Complex Formation with C1 Inhibitor Are Linked to the Severity of Hereditary Angioedema PubMed: 26371246
J Neuroinflammation.  Mannose-binding lectin-associated serine protease 2 (MASP-2) contributes to poor disease outcome in humans and mice with pneumococcal meningitis PMC5234106
Frontiers in Immunology AmpliSeq screening of genes encoding the C-type lectin receptors and their signaling components reveals a common variant in MASP1 associated with … Pubmed:29515573
Journal of Proteomics Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approach Pubmed: 30605725
Kidney International Reports Clinical value of complement activation biomarkers in overt diabetic nephropathy
Kidney International Reports Comparison of Complement Pathway Activation in Autoimmune Glomerulonephritis Pubmed:35571000
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB895Mu02 Recombinant Mannose Associated Serine Protease 1 (MASP1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB895Mu01 Recombinant Mannose Associated Serine Protease 1 (MASP1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB895Mu02 Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) WB; IHC; ICC; IP.
PAB895Mu01 Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) WB,IHC
SEB895Mu ELISA Kit for Mannose Associated Serine Protease 1 (MASP1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB895Mu Multiplex Assay Kit for Mannose Associated Serine Protease 1 (MASP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.