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Multiplex Assay Kit for Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay)

SORD; L-iditol 2-dehydrogenase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-105 101
EDTA plasma(n=5) 81-98 87
heparin plasma(n=5) 79-90 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-94% 94-105% 97-104% 94-102%
EDTA plasma(n=5) 83-101% 79-101% 95-103% 91-98%
heparin plasma(n=5) 87-101% 86-101% 80-92% 83-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SDH) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Kafkas Univ Vet Fak Derg The Importance of Concentrations of Sorbitol Dehydrogenase and Glutamate Dehydrogenase and B-Mode Ultrasonographic Examination in The Diagnosis of Hepatic Lipidosis in Dairy Cows Kafkas: Source
Journal of Food Science and Technology Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats Springer: Source
Clinical and Experimental Optometry Aldo-keto reductase and sorbitol dehydrogenase enzymes in Egyptian diabetic patients with and without proliferative diabetic retinopathy. Pubmed: 23452182
Biomedicine & Pharmacotherapy Zinc/alogliptin combination attenuates testicular toxicity induced by doxorubicin in rats: Role of oxidative stress, apoptosis and TGF-β1/NF-κB signaling pubmed:29091894
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Effect of the combination between empagliflozin and calcipotriol on cadmium-induced testicular toxicity in rats
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPB495Bo01 Recombinant Sorbitol Dehydrogenase (SDH) Positive Control; Immunogen; SDS-PAGE; WB.
PAB495Bo01 Polyclonal Antibody to Sorbitol Dehydrogenase (SDH) WB; IHC; ICC; IP.
MAB495Bo21 Monoclonal Antibody to Sorbitol Dehydrogenase (SDH) WB; IHC; ICC; IP.
SEB495Bo ELISA Kit for Sorbitol Dehydrogenase (SDH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB495Bo Multiplex Assay Kit for Sorbitol Dehydrogenase (SDH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.